Uyini Umehluko Phakathi Kwe-Low Speed ​​Centrifuges kanye ne-High Speed ​​Centrifuges

Ama-Centrifuge angamathuluzi abalulekile kumalabhorethri, anikeza ukuhlukaniswa kwesampula okusebenzayo okusekelwe emigomeni ye-sedimentation kanye ne-centrifugal force. Umehluko owodwa obalulekile phakathi kwama-centrifuges usejubaneni lawo lokusebenza—isivinini esiphansi se-centrifuge futhi isivinini esikhulu se-centrifuge. Ukuqonda umehluko phakathi kwalezi zinhlobo ezimbili zama-centrifuge kubalulekile kubacwaningi nososayensi abafuna imishini efaneleke kakhulu yezinhlelo zabo zokusebenza ezithile.

I-Low Speed ​​​​Centrifuges:

1.Uhambo lokusheshisa: Ama-centrifuge esivinini esiphansi ngokuvamile asebenza ngokuphenduka okuphansi ngomzuzu (RPM) uma kuqhathaniswa nozakwabo besivinini esikhulu. Ngokuvamile zisuka kumakhulu ambalwa ziye ezinkulungwaneni ezimbalwa ze-RPM.

2.I-Relative Centrifugal Force (RCF): I-RCF ekhiqizwe ama-centrifuges esivinini esiphansi imaphakathi, iwenza afanelekele izinhlelo zokusebenza ezidinga ukuhlukaniswa okuthambile kwamasampuli.

3.Izicelo:

● Ukuxilongwa Komtholampilo: Kulungele imisebenzi evamile njengokuhlukanisa izingxenye zegazi kumalabhorethri omtholampilo.

● I-Cell Culture: Isetshenziselwa ukugaya amaseli nokuhlukanisa izingxenye zamaselula ngaphandle kokudala umonakalo.

● Umsebenzi Ojwayelekile Waselabhorethri: Ifaneleka kahle ukulungiselela isampula evamile kanye nezinqubo eziyisisekelo zokuhlukanisa.

4.Amandla wesampula: Ama-centrifuge esivinini esiphansi ngokuvamile athatha umthamo wesampula ephezulu, okuwenza afanelekele ukucubungula amavolumu amakhulu.

I-High Speed ​​​​Centrifuges:

1.Uhambo lokusheshisa: Ama-centrifuge anesivinini esiphezulu asebenza ku-RPM ephakeme kakhulu, ngokuvamile afinyelela emashumini ezinkulungwane kuya ngaphezu kwezinkulungwane eziyikhulu RPM.

2.I-RCF: Amanani aphezulu e-RCF akhiqizwa yilawa ma-centrifuges anika amandla ukuhlukaniswa okuphumelelayo kwezinhlayiya, kuhlanganise nezingxenye ezincane zeselula.

3.Izicelo:

● Ucwaningo Lwebhayoloji Yamangqamuzana: Isetshenziselwa i-DNA, i-RNA, ukuhlukaniswa kwamaprotheni kanye nokuzihlukanisa.

● I-Biochemistry: Ibalulekile ezinhlelweni ezidinga ukuhlanzwa kwama-biomolecules.

● Ucwaningo Lwezokwelapha Nolwemithi: Kusetshenziswa ezifundweni ezibandakanya ukulungiswa kwesampula okuthuthukisiwe nokuhlaziya.

4.Amandla wesampula: Ama-centrifuge esivinini esikhulu ngokuvamile anesampula esiphansi somthamo uma kuqhathaniswa namamodeli anesivinini esiphansi, alungele ukucubungula amavolumu amancane amasampuli.

Ukukhetha i-Centrifuge efanele:

Imigomo Yokuhlola: Cabangela izidingo ezithile zokuhlola kwakho. Ama-centrifuge anejubane eliphansi afanele ukuhlukaniswa okuthambile, kuyilapho ama-centrifuge anesivinini esiphezulu elungele izinqubo eziyinkimbinkimbi ezidinga amandla aphezulu.

Izici zesampula: Hlola imvelo yamasampuli akho. Amasampula athambile angase azuze ekuhlukaniseni okuthambile okunikezwa ama-centrifuge anesivinini esiphansi, kuyilapho ama-centrifuge esivinini esikhulu esebenza kahle ekucubunguleni izinto eziminyene.

Umsebenzi weLabhorethri: Hlola ivolumu kanye nemvamisa yokucubungula isampula elabhorethri yakho. Ama-centrifuge anesivinini esiphansi asebenza kahle emisebenzini evamile, kuyilapho ama-centrifuge anesivinini esiphezulu enza kahle kakhulu ezinhlelweni zocwaningo ezithuthukisiwe.

Sengiphetha, ukukhetha phakathi kwama-centrifuge anesivinini esiphansi kanye nesivinini esikhulu kuncike ezidingweni ezithile zokuhlolwa kwakho. Kungakhathaliseki ukuthi usebenza ekulungiseleleni isampula evamile noma wenza ucwaningo oluyinkimbinkimbi lwebhayoloji yamangqamuzana, ukuqonda umehluko phakathi kwalawa ma-centrifuge kubalulekile ekuthuthukiseni izinqubo zaselabhorethri kanye nokuthola imiphumela ethembekile.